LOCUS       pLV-[hsa-miR-99a]        9135 bp    DNA     circular     11-APR-2007
SOURCE      
COMMENT     
COMMENT     This file is created by Biosettia Inc.
            http://www.biosettia.com/
FEATURES             Location/Qualifiers
     misc_feature    1807..1929
                     /label=cPPT
     CDS             7056..7916
                     /label=Amp(R)
     promoter        8117..8657
                     /label=pUC ori
     promoter        7..235
                     /label=RSV
     LTR             5661..5895
                     /label=3' LTR
     intron          526..1690
                     /label=Intron
     rep_origin      complement(6470..6925)
                     /label=f1 ori
     LTR             236..416
                     /label=5'LTR
     polyA_signal    5967..6097
                     /label=SV40 pA
     misc_feature    6098..6311
                     /label=SV40 ori
     misc_feature    4982..5578
                     /label=WPRE
     promoter        1933..2328
                     /label=human EF1a promoter
     intron          2361..3600
                     /label=intron
     CDS             3647..4972
                     /label=rPuro (RFP Puro)
     misc_RNA        3110..3390
                     /label=hsa-mir-99a precursor
BASE COUNT     2184 a      2356 c      2457 g      2138 t 
ORIGIN
        1 aagcttaatg tagtcttatg caatactctt gtagtcttgc aacatggtaa cgatgagtta 
       61 gcaacatgcc ttacaaggag agaaaaagca ccgtgcatgc cgattggtgg aagtaaggtg 
      121 gtacgatcgt gccttattag gaaggcaaca gacgggtctg acatggattg gacgaaccac 
      181 tgaattgccg cattgcagag atattgtatt taagtgccta gctcgataca taaacgggtc 
      241 tctctggtta gaccagatct gagcctggga gctctctggc taactaggga acccactgct 
      301 taagcctcaa taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga 
      361 ctctggtaac tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagtgg 
      421 cgcccgaaca gggacttgaa agcgaaaggg aaaccagagg agctctctcg acgcaggact 
      481 cggcttgctg aagcgcgcac ggcaagaggc gaggggcggc gactggtgag tacgccaaaa 
      541 attttgacta gcggaggcta gaaggagaga gatgggtgcg agagcgtcag tattaagcgg 
      601 gggagaatta gatcgcgatg ggaaaaaatt cggttaaggc cagggggaaa gaaaaaatat 
      661 aaattaaaac atatagtatg ggcaagcagg gagctagaac gattcgcagt taatcctggc 
      721 ctgttagaaa catcagaagg ctgtagacaa atactgggac agctacaacc atcccttcag 
      781 acaggatcag aagaacttag atcattatat aatacagtag caaccctcta ttgtgtgcat 
      841 caaaggatag agataaaaga caccaaggaa gctttagaca agatagagga agagcaaaac 
      901 aaaagtaaga ccaccgcaca gcaagcggcc gctgatcttc agacctggag gaggagatat 
      961 gagggacaat tggagaagtg aattatataa atataaagta gtaaaaattg aaccattagg 
     1021 agtagcaccc accaaggcaa agagaagagt ggtgcagaga gaaaaaagag cagtgggaat 
     1081 aggagctttg ttccttgggt tcttgggagc agcaggaagc actatgggcg cagcgtcaat 
     1141 gacgctgacg gtacaggcca gacaattatt gtctggtata gtgcagcagc agaacaattt 
     1201 gctgagggct attgaggcgc aacagcatct gttgcaactc acagtctggg gcatcaagca 
     1261 gctccaggca agaatcctgg ctgtggaaag atacctaaag gatcaacagc tcctggggat 
     1321 ttggggttgc tctggaaaac tcatttgcac cactgctgtg ccttggaatg ctagttggag 
     1381 taataaatct ctggaacaga tttggaatca cacgacctgg atggagtggg acagagaaat 
     1441 taacaattac acaagcttaa tacactcctt aattgaagaa tcgcaaaacc agcaagaaaa 
     1501 gaatgaacaa gaattattgg aattagataa atgggcaagt ttgtggaatt ggtttaacat 
     1561 aacaaattgg ctgtggtata taaaattatt cataatgata gtaggaggct tggtaggttt 
     1621 aagaatagtt tttgctgtac tttctatagt gaatagagtt aggcagggat attcaccatt 
     1681 atcgtttcag acccacctcc caaccccgag gggacccgac aggcccgaag gaatagaaga 
     1741 agaaggtgga gagagagaca gagacagatc cattcgatta gtgaacggat ctcgacggta 
     1801 tcggttaact tttaaaagaa aaggggggat tggggggtac agtgcagggg aaagaatagt 
     1861 agacataata gcaacagaca tacaaactaa agaattacaa aaacaaatta caaaattcaa 
     1921 aattttatcg atgcctcccc gtcaccaccc cccccaaccc gccccgaccg gagctgagag 
     1981 taattcatac aaaaggactc gcccctgcct tggggaatcc cagggaccgt cgttaaactc 
     2041 ccactaacgt agaacccaga gatcgctgcg ttcccgcccc ctcacccgcc cgctctcgtc 
     2101 atcactgagg tggagaagag catgcgtgag gctccggtgc ccgtcagtgg gcagagcgca 
     2161 catcgcccac agtccccgag aagttggggg gaggggtcgg caattgaacc ggtgcctaga 
     2221 gaaggtggcg cggggtaaac tgggaaagtg atgtcgtgta ctggctccgc ctttttcccg 
     2281 agggtggggg agaaccgtat ataagtgcag tagtcgccgt gaacgttctt tttcgcaacg 
     2341 ggtttgccgc cagaacacag gtaagtgccg tgtgtggttc ccgcgggcct ggcctcttta 
     2401 cgggttatgg cccttgcgtg ccttgaatta cttccacgcc cctggctgca gtacgtgatt 
     2461 cttgatcccg agcttcgggt tggaagtggg tgggagagtt cgaggccttg cgcttaagga 
     2521 gccccttcgc ctcgtgcttg agttgaggcc tggcctgggc gctggggccg ccgcgtgcga 
     2581 atctggtggc accttcgcgc ctgtctcgct gctttcgata agtctctagc catttaaaat 
     2641 ttttgatgat atcctgcgac gctttttttc tggcaagata gtcttgtaaa tgcgggccaa 
     2701 gatctgcaca ctggtatttc ggtttttggg gccgcgggcg gcgacggggc ccgtgcgtcc 
     2761 cagcgcacat gttcggcgag gcggggcctg cgagcgcggc caccgagaat cggacggggg 
     2821 tagtctcaag ctggccggcc tgctctggtg cctggcctcg cgccgccgtg tatcgccccg 
     2881 ccctgggcgg caaggctggc ccggtcggca ccagttgcgt gagcggaaag atggccgctt 
     2941 cccggccctg ctgcagggag ctcaaaatgg aggacgcggc gctcgggaga gcgggcgggt 
     3001 gagtcaccca cacaaaggaa aagggccttt ccgtcctcag ccgtcgcttc atgtgactcc 
     3061 acggagtacc gggcgccgtc caggcacctc gattagttct cgaggatcca aaacatttat 
     3121 actaaaggat caatattatt tttgactctt aattgcatca gatatttaca acaaattcta 
     3181 tattaatagg gggcccatgc aagatgttgc ccattggcat aaacccgtag atccgatctt 
     3241 gtggtgaagt ggaccgcaca agctcgcttc tatgggtctg tgtcagtgtg gtaatctgac 
     3301 aaaatgctat acacagtgcc gttcaacaat agttcagtaa aatcctgtta aactccagtt 
     3361 gattatatac ttttgaagtc attatatttt gctagctcga gcttttggag tacgtcgtct 
     3421 ttaggttggg gggaggggtt ttatgcgatg gagtttcccc acactgagtg ggtggagact 
     3481 gaagttaggc cagcttggca cttgatgtaa ttctccttgg aatttgccct ttttgagttt 
     3541 ggatcttggt tcattctcaa gcctcagaca gtggttcaaa gtttttttct tccatttcag 
     3601 gtgtcgtgaa aactacccct ctagagtcga gctaccggtc gccaccatgg tgagcaaggg 
     3661 cgaggaggat aacatggcca tcatcaagga gttcatgcgc ttcaaggtgc acatggaggg 
     3721 ctccgtgaac ggccacgagt tcgagatcga gggcgagggc gagggccgcc cctacgaggg 
     3781 cacccagacc gccaagctga aggtgaccaa gggtggcccc ctgcccttcg cctgggacat 
     3841 cctgtcccct cagttcatgt acggctccaa ggcctacgtg aagcaccccg ccgacatccc 
     3901 cgactacttg aagctgtcct tccccgaggg cttcaagtgg gagcgcgtga tgaacttcga 
     3961 ggacggcggc gtggtgaccg tgacccagga ctcctccctg caggacggcg agttcatcta 
     4021 caaggtgaag ctgcgcggca ccaacttccc ctccgacggc cccgtaatgc agaagaagac 
     4081 catgggctgg gaggcctcct ccgagcggat gtaccccgag gacggcgccc tgaagggcga 
     4141 gatcaagcag aggctgaagc tgaaggacgg cggccactac gacgctgagg tcaagaccac 
     4201 ctacaaggcc aagaagcccg tgcagctgcc cggcgcctac aacgtcaaca tcaagttgga 
     4261 catcacctcc cacaacgagg actacaccat cgtggaacag tacgaacgcg ccgagggccg 
     4321 ccactccacc ggcggcatgg acgagctgta caaggaccca ccggtcgcca ccatgaccga 
     4381 gtacaagccc acggtgcgcc tcgccacccg cgacgacgtc cccagggccg tacgcaccct 
     4441 cgccgccgcg ttcgccgact accccgccac gcgccacacc gtcgatccgg accgccacat 
     4501 cgagcgggtc accgagctgc aagaactctt cctcacgcgc gtcgggctcg acatcggcaa 
     4561 ggtgtgggtc gcggacgacg gcgccgcggt ggcggtctgg accacgccgg agagcgtcga 
     4621 agcgggggcg gtgttcgccg agatcggccc gcgcatggcc gagttgagcg gttcccggct 
     4681 ggccgcgcag caacagatgg aaggcctcct ggcgccgcac cggcccaagg agcccgcgtg 
     4741 gttcctggcc accgtcggcg tctcgcccga ccaccagggc aagggtctgg gcagcgccgt 
     4801 cgtgctcccc ggagtggagg cggccgagcg cgccggggtg cccgccttcc tggagacctc 
     4861 cgcgccccgc aacctcccct tctacgagcg gctcggcttc accgtcaccg ccgacgtcga 
     4921 gtgcccgaag gaccgcgcga cctggtgcat gacccgcaag cccggtgcct gagcggccgc 
     4981 gtcgacaatc aacctctgga ttacaaaatt tgtgaaagat tgactggtat tcttaactat 
     5041 gttgctcctt ttacgctatg tggatacgct gctttaatgc ctttgtatca tgctattgct 
     5101 tcccgtatgg ctttcatttt ctcctccttg tataaatcct ggttgctgtc tctttatgag 
     5161 gagttgtggc ccgttgtcag gcaacgtggc gtggtgtgca ctgtgtttgc tgacgcaacc 
     5221 cccactggtt ggggcattgc caccacctgt cagctccttt ccgggacttt cgctttcccc 
     5281 ctccctattg ccacggcgga actcatcgcc gcctgccttg cccgctgctg gacaggggct 
     5341 cggctgttgg gcactgacaa ttccgtggtg ttgtcgggga agctgacgtc ctttccatgg 
     5401 ctgctcgcct gtgttgccac ctggattctg cgcgggacgt ccttctgcta cgtcccttcg 
     5461 gccctcaatc cagcggacct tccttcccgc ggcctgctgc cggctctgcg gcctcttccg 
     5521 cgtcttcgcc ttcgccctca gacgagtcgg atctcccttt gggccgcctc cccgcctgga 
     5581 attcgagctc ggtaccttta agaccaatga cttacaaggc agctgtagat cttagccact 
     5641 ttttaaaaga aaagggggga ctggaagggc taattcactc ccaacgaaga caagatctgc 
     5701 tttttgcttg tactgggtct ctctggttag accagatctg agcctgggag ctctctggct 
     5761 aactagggaa cccactgctt aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt 
     5821 gtgcccgtct gttgtgtgac tctggtaact agagatccct cagacccttt tagtcagtgt 
     5881 ggaaaatctc tagcagtagt agttcatgtc atcttattat tcagtattta taacttgcaa 
     5941 agaaatgaat atcagagagt gagaggaact tgtttattgc agcttataat ggttacaaat 
     6001 aaagcaatag catcacaaat ttcacaaata aagcattttt ttcactgcat tctagttgtg 
     6061 gtttgtccaa actcatcaat gtatcttatc atgtctggct ctagctatcc cgcccctaac 
     6121 tccgcccatc ccgcccctaa ctccgcccag ttccgcccat tctccgcccc atggctgact 
     6181 aatttttttt atttatgcag aggccgaggc cgcctcggcc tctgagctat tccagaagta 
     6241 gtgaggaggc ttttttggag gcctagggac gtacccaatt cgccctatag tgagtcgtat 
     6301 tacgcgcgct cactggccgt cgttttacaa cgtcgtgact gggaaaaccc tggcgttacc 
     6361 caacttaatc gccttgcagc acatccccct ttcgccagct ggcgtaatag cgaagaggcc 
     6421 cgcaccgatc gcccttccca acagttgcgc agcctgaatg gcgaatggga cgcgccctgt 
     6481 agcggcgcat taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc 
     6541 agcgccctag cgcccgctcc tttcgctttc ttcccttcct ttctcgccac gttcgccggc 
     6601 tttccccgtc aagctctaaa tcgggggctc cctttagggt tccgatttag tgctttacgg 
     6661 cacctcgacc ccaaaaaact tgattagggt gatggttcac gtagtgggcc atcgccctga 
     6721 tagacggttt ttcgcccttt gacgttggag tccacgttct ttaatagtgg actcttgttc 
     6781 caaactggaa caacactcaa ccctatctcg gtctattctt ttgatttata agggattttg 
     6841 ccgatttcgg cctattggtt aaaaaatgag ctgatttaac aaaaatttaa cgcgaatttt 
     6901 aacaaaatat taacgcttac aatttaggtg gcacttttcg gggaaatgtg cgcggaaccc 
     6961 ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct 
     7021 gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg 
     7081 cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg 
     7141 tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc 
     7201 tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca 
     7261 cttttaaagt tctgctatgt ggcgcggtat tatcccgtat tgacgccggg caagagcaac 
     7321 tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa 
     7381 agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg 
     7441 ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt 
     7501 ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg 
     7561 aagccatacc aaacgacgag cgtgacacca cgatgcctgt agcaatggca acaacgttgc 
     7621 gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga 
     7681 tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta 
     7741 ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc 
     7801 cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg 
     7861 atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt 
     7921 cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa 
     7981 ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt 
     8041 cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt 
     8101 ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt 
     8161 tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga 
     8221 taccaaatac tgttcttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag 
     8281 caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata 
     8341 agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg 
     8401 gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga 
     8461 gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca 
     8521 ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa 
     8581 acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt 
     8641 tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac 
     8701 ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt 
     8761 ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga 
     8821 ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cccaatacgc aaaccgcctc 
     8881 tccccgcgcg ttggccgatt cattaatgca gctggcacga caggtttccc gactggaaag 
     8941 cgggcagtga gcgcaacgca attaatgtga gttagctcac tcattaggca ccccaggctt 
     9001 tacactttat gcttccggct cgtatgttgt gtggaattgt gagcggataa caatttcaca 
     9061 caggaaacag ctatgaccat gattacgcca agcgcgcaat taaccctcac taaagggaac 
     9121 aaaagctgga gctgc 
//
